Fig. 4

α5-nAChR mediates PD-L1 expression via STAT3 binding to the PD-L1 or Jab1 promoter in LUAD cells. A, B pSTAT3 expression in the whole, nuclear, and cytoplasmic lysates of A549 cells and H1299 cells. C, D STAT3 bound to the PD-L1 and Jab1 promoter in A549 cells. E, F Silencing STAT3 downregulated PD-L1 and Jab1 expression in Α549 cells and H1299 cells. Data are presented as the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ns not significantly different