Fig. 5
From: Lamprey immune protein triggers the ferroptosis pathway during zebrafish embryonic development
LIP overexpression in zebrafish larvae causes edema by triggering ferroptosis. A Ferroptosis expression in four stages of embryonic development of zebrafish was confirmed on Western Blot. B-D Zebrafish whole embryo iron (B), MDA (C), T-GSH and GSH/GSSG ratio (D) detection at 19, 36, 60 and 96hpf. E Representative phenotype of the larvae categorized into 4 groups: P1 = no edema, P2 = mild edema, P3 = severe edema, P4 = very severe edema. F Effects of vitamin E on survival rate of overexpressed LIP zebrafish. G Phenotypic classification and representative images of zebrafish larvae treated with vitamin at 96 hpf. H Phenotypic classification of 96hpf larvae treated by combination of FAC and DFO. I Transmission electron microscopy performed on heart sections from zebrafish myocardium demonstrates abnormal edema and mitochondria ultrastructure in LIP overexpression hearts. Pink: cell nucleus, blue: normal mitochondria, red: mitochondria after ferroptosis. Scale bar 2 μm. Data were given as means ± standard deviation. All figures are representative of three biological replicates (per test n = 100). ***, P < 0.001; **, P < 0.01; *, 0.01 < P < 0.05