Fig. 3
From: Lamprey immune protein triggers the ferroptosis pathway during zebrafish embryonic development
LIP inhibits embryonic development of transgenic zebrafish by inducing cell death. A Heart rate of 48-120hpf zebrafish every 20 s. B LIP overexpression induces effective cell death in whole embryos and heart. Zebrafish embryos were stained with propidium iodide (PI) at 24, 48, 72 and 96hpf. Death cells are visible as bright red spots, and less bright homogenous red staining, an unspecific background staining. Naive zebrafish exhibited few or no death cells in whole organism. In contrast, significantly increased staining was observed throughout the hearts in LIP overexpression embryos (white arrows). Scale bar is 500 μm. C Quantification of death particle number in whole embryo shows increase in LIP overexpression embryos at 24, 48, 72 and 96hpf. D Quantification of death particle number in heart shows increase in LIP overexpression embryos at 24, 48, 72 and 96hpf. Data were given as means ± standard deviation. All figures are representative of three biological replicates (per test n = 10). ***, P < 0.001; **, P < 0.01; *, 0.01 < P < 0.05