Fig. 2
From: Lamprey immune protein triggers the ferroptosis pathway during zebrafish embryonic development
Overexpression of lip inhibits embryonic development of zebrafish. A The Keplan-meier survival curves of larvae (n = 100). P-values were obtained from two-sided log-rank tests. B The malformation rates at 4-7dpf (n = 100) of Dox-treated embryos were calculated and compared with those of their corresponding controls. C LIP overexpression causes 7dpf zebrafish to develop malformations. D The ratio of various malformations of 7dpf zebrafish (n = 100). E HE staining of zebrafish larvae with LIP overexpression malformation. F Lateral view of zebrafish with measurements of body length, eyeball diameter and yolk sac area. G-I Body lengths (G), eyeball diameters (H) and yolk sac area (I) of embryos injected with Tg(TRE:EGFP-lip) or naive control at 4 and 7dpf (n = 10). Data were given as means ± standard deviation. All figures are representative of three biological replicates. **, P < 0.01; *, 0.01 < P < 0.05. Scale bar is 250 μm