Fig. 1
From: Lamprey immune protein triggers the ferroptosis pathway during zebrafish embryonic development
Establishment of a LIP-overexpression transgenic zebrafish model. A The plasmid was microinjected into fertilized eggs at one-cell stage. The positive F0 fish was individually crossed with naive fish to obtain positive F1 fish. F2 fish was reproduced by selfing the positive F1. Imaging EGFP expression in F2 embryos with or without Dox was performed using a fluorescence microscope. B Confocal imaging overexpression of fluorescent expression of LIP zebrafish. Scale 50 μm. C Positive F2 individuals were obtained by PCR screening. M: DNA ladder; lanes a# and b#: individual DNA samples (two transgenic lines with fluorescent expression obtained). The positive samples amplified 367–374 bp DNA bands. D Western blot analysis of LIP and EGFP in F2 fish. E Fluorescent expression efficiency of F2 and F3 generation transgenic zebrafish. Scale bar is 500 μm