Fig. 5
Morphogenetic pathway analysis in syndromic MB and associated neoplasms. A, B Heatmap of overexpressed genes from the Shh (selected genes) (A) and Hippo (B) pathways. WWTR1 is also known as TAZ, and is the transcription factor that partners with YAP and TEAD-family or RUNX-family transcription factors for the activation of the Hippo pathway. VGLL3 is also a cofactor for the TEAD-family transcription factors. Note overexpression of these transcription factors in the FAP-associated desmoid fibromatosis (Desm), and MB-WNT, and also the Li-Fraumeni high-grade (HG) sarcoma. Pit, pituitary adenoma/prolactinoma. C–E The growth pathways Wnt-β-catenin (C), TGFβ (D) and ERK/MAPK (E) are represented by box-and-whiskers plots. Significant differences are shown with red asterisks: ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns, not significant. The tumor order is aligned to (A) labels. F Hormone specification pathways show the prolactin (PRL) secretory program massively activated in M6 atypical prolactinoma. F RTK fold-overexpression heatmap. The RTKs boxed in red or blue show overexpression levels significantly higher or in the 90th percentile of expression values from the MG-eDB1 expression database (see Methods), respectively