Fig. 2
From: THZ1 targeting CDK7 suppresses c-KIT transcriptional activity in gastrointestinal stromal tumours
Knockdown of CDK7 decreases cell viability and proliferation and induces cell cycle arrest. A Immunoblotting analysis of CDK7 expression after targeting siRNA-mediated CDK7 knockdown in GIST-T1 and GIST-882 cells. A nontargeting siRNA and two independent siRNAs (siRNA1 and siRNA2) are represented by siNC, siCDK7-1, and siCDK7-2. B CCK-8 cell viability assay after CDK7 knockdown in GIST-T1 and GIST-882 cells. C Colony formation assays of GIST-T1 and GIST-882 cells after CDK7 knockdown. D Flow cytometry analysis was used to detect and analyse the cell cycle distribution after CDK7 knockdown. E Immunoblotting analysis of cyclin-D1, CDK4 and γH2AX expression after CDK7 knockdown