Fig. 8

Effects of endocytic and exocytotic inhibitors on filopodia prevalence after Ca²⁺ readdition. Results shown are representative of 4–9 experiments. a–d Filopodia prevalence after Ca²⁺ alone (Ca²⁺ alone) or in the presence of various inhibitors, a, b Ca²⁺ readdition in the presence or absence of 0.4 M sucrose or 48 µM dynasore. a Significance by ANOVA, P = 0.153, b significance by ANOVA, P = 0.0034, *P = 0.038. c, d Ca²⁺ readdition in the presence or absence of 25 mM KCl with and without nifedipine or 25 mM KCl in Ca²⁺-free HBSS (Ca²⁺-free). c Significance by ANOVA, P = 0.049, **P = 0.033. d Significance by ANOVA, P = 0.022, ***P = 0.012. e, f Samples pretreated during ER depletion with or without brefeldin (20 µM) followed by Ca²⁺ readdition. e ^†Treatments differ at P = 0.044