Fig. 4
TRPC1 on microtubules and filopodia. a Linear arrays of TRPC1 (green) are aligned parallel to features containing STIM1 (red). Inset: enlargement of area designated by arrowhead, showing features side by side. b TRPC1 loci aligned on filopodia (white arrowheads). c TRPC1 loci (green arrowheads) alternating with STIM1 (red) during Ca2+ readdition. d Colocalization of β-tubulin (left panel) and TRPC1 (middle panel) in an untreated cell. TRPC1 colocalizes with microtubules bordering the edge (arrowheads) and in the interior (arrows). Panel at right is the overlay. e Microtubules (red) labelled by antibody against β-tubulin penetrate to the cell edge but do not enter filopodia (white arrowheads) in an untreated cell. The colocalization of TRPC1 and β-tubulin is apparent in higher parts of the cell (black arrow). Inset: TRPC1 loci (green) free of microtubules (red) at the cell edge. f Colocalization of β-tubulin (upper frame) and TRPC1 (lower frame) shows that TRPC1 spots (green arrowheads) extend beyond the ends of microtubules. Interior to the edge, TRPC1 is found in diffuse areas around the microtubules (white arrow). Bars = 10 µm