Fig. 7
From: CCM signaling complex (CSC) couples both classic and non-classic Progesterone receptor signaling
Overlapped Differentially Expressed Genes (DEGs)/Proteins (DEPs) using systems biology approaches. Gene overlaps were calculated at both the A translational, B transcriptional, and c both transcriptional and translational levels to elucidate any shared signaling pathways between treatment groups. Proteomic overlaps (panel A) are demonstrated for both CSC disturbance as well as hormone treatments. Individual comparisons between hormone treatments and CCM knockdowns are illustrated, and the combined knockdowns (disrupted CSC) compared to hormone treatments. Transcriptomic overlaps (panel B) are demonstrated for hormone treatments. Individual comparisons between hormone treatments are illustrated as well as the combined hormone treatments comparison. Pathway classification and functional enrichment was performed on overlapped genes at both the proteome and transcriptome levels to evaluate any shared signaling mechanisms altered through a disturbed CSC and hormone treatments (panel C). For all analysis, PRG-only comparisons (proteome and transcriptome) were conducted using published proteomic/transcriptomic databases to assemble a working database that could be compared to our MIF and MIF + PRG treated samples. If available, fold changes and p-values were obtained from the databases to increase the depth of analysis of the multi-omics comparison. Raw data can be provided upon request