Fig. 5
From: CCM signaling complex (CSC) couples both classic and non-classic Progesterone receptor signaling
CCM2 is a cornerstone for the essential stability of the CSC. Luminal-A T47D breast cancer cells were treated with siRNA targeting members of the CSC, followed by measurement of their corresponding RNA/protein expression levels. A Silencing of CCM2 decreases the expression level of both CCM1/3 proteins in T47D cells. T47D cells were treated to silence all three CCMs (1, 2 or 3) for 48 h (Left upper and lower panels). The relative expression levels of CCM1/3 and three major isoforms of CCM2 proteins were measured through quantification of band intensities and normalized against β-actin (ACTB) followed by SC controls, (right panel, n = 3). B Significantly increased RNA levels of CCM2 isoforms in silenced CCM1 (CCM1-KD) and CCM3 (CCM3-KD) T47D cells. T47D cells were treated to silence all three CCMs (1, 2 or 3) for 48 h and the relative RNA expression levels of CCM1, CCM3, and 5 isoforms of CCM2 were measured by RT-qPCR (Fold, n = 3). C The summarized CmPn signaling network among the CSC, nPRs, and mPRs under mPR-specific PRG actions for Luminal-A breast cancer tumorigenesis. This diagram details the relationships within the novel CSC-mPRs-PRG-nPRs (CmPn) signaling network, under mPR-specific PRG actions in Luminal-A breast cancer cells. Yellow line separates transcriptional and translational levels. The + symbols represent enhancement,—symbols represent inhibition while ± symbols represent various regulation for the expression of targeted genes/proteins. Red-colored symbols represent positive effects of mPR-specific PRG actions, blue-colored symbols/lines represent negative effects of treatment, while purple-colored symbols/lines represent variable effects. Dark green-colored letters indicate the direct supporting data generated from this work. Arrows indicate effect direction, solid line is the direct impact, dotted line for indirect effects. In all bar plots, red line is the control baseline for fold change measurements (−/+). *, **, *** above bar indicates P ≤ 0.05, 0.01, or 0.001, respectively, for paired t-test