Fig. 6
From: Androgen receptor variant-7 regulation by tenascin-c induced src activation
Activated Src regulates post-translational stability and alternative splicing of AR-V7. A RT-qPCR analysis on the impact of Src knockdown using siRNA on AR-V7 and TNC expression in both VCaP and 22Rv1 cultured on BSA or TNC. B Western blot analysis on Src knockdown in VCaP and 22Rv1 cultured on BSA and TNC. siRNA knockdown of Src does not affect TNC-induced FAK activation but destabilizes AR-V7. C Western blot analysis of Src kinase inhibition using PP1with DMSO as the vehicle control on AR-V7, AR-FL, and TNC protein level. D RT-qPCR analysis on AR-FL, AR-V7, and TNC expression followed by Src kinase inhibition using PP1 inhibitor All data represent mean ± SD analyzed by unpaired students t-test (n = 3) *p‹0.05, ** p < 0.01, ***p < 0.001, ****p < 0.0001