Fig. 5
From: Androgen receptor variant-7 regulation by tenascin-c induced src activation
TNC-induced post-translational stability of AR-V7 is not mediated by Integrin mediated FAK activation. A RT-qPCR analysis reveal that VCaP and 22Rv1 express integrin αv/2/9 and β1. B RT-qPCR analysis on expression of AR-V7, TNC, and AR-FL in VCaP and 22Rv1 cultured on BSA or TNC after integrin β1 knockdown using siRNA C Western blot analysis on the impact of integrin β1 knockdown on TNC signaling in VCaP and 22Rv1. SiRNA knockdown of integrin β1 impedes TNC-induced FAK activation but does not affect Src activation nor AR-V7 post-translational stability. All data represent mean ± SD analyzed by unpaired students t-test (n = 3); ** p < 0.01; ***p < 0.001; ****p < 0.0001