Fig. 2
From: Androgen receptor variant-7 regulation by tenascin-c induced src activation
TNC and AR-V7 expression decrease in 3D heterotypical organoids treated with testosterone. A and B Effect of testosterone (1 nM), estradiol (10 nM), and enzalutamide (10 µM) on TNC, AR-V7, AR-FL, and Kallikrein related peptidase 3 (KLK3)/ Prostate Specific antigen (PSA) in 3D prostate cancer (VCaP or 22Rv1) and heterotypical (VCaP-22Rv1/MC3T3-E1) organoids using RT-qPCR. Human-specific primers were used for RT-qPCR to exclude transcripts from mouse derived preosteoblasts (MC3T3-E1). All data represent mean ± SD analyzed by unpaired students t-test (n = 3) *p < 0.05; ** p < 0.01; ***p < 0.001; ****p < 0.0001