Fig. 1
From: Androgen receptor variant-7 regulation by tenascin-c induced src activation
TNC deposition in regions of contact between mouse preosteoblasts and prostate cancer cells in 3D heterotypical organoids. A Compartmentalization of preosteoblasts (RFP) and prostate cancer cells (VCaP and 22Rv1-GFP) in 3D heterotypical organoids (Scale bars, 10x: 100 µm and 20x:50 µm). B TNC (FITC) expression by preosteoblasts in 3D organoids (Scale bars, 10x:100 µm and 20x:50 µm). Nuclei are counterstained with DAPI. C TNC staining (FITC) on 3D heterotypical organoids composed of VCaP and 22Rv1 (yellow arrow: depicted with DAPI) and MC3T3-E1 (RFP) (Scale bar, 10x: 100 µm). D 3D reconstruction using Imaris of 20 × confocal images of 3D heterotypical organoids depicting TNC deposition (green-white arrow) on regions of contact between prostate cancer cells (DAPI) and MC3T3-E1 (RFP) (Scale bars, 20x:50 µm, Imaris: 20 µm)