Fig. 4
From: MST4 negatively regulates type I interferons production via targeting MAVS-mediated pathway
The interaction between MST4 and MAVS. A Immunoblot analysis of lysates of 293 T cells transfected with Flag-MDA5, Flag-RIG-I, or Flag-MAVS along with Myc-MST4 for 24 h, followed by immunoprecipitation with anti-Flag beads. B Immunoblot analysis of lysates of 293 T cells infected with SeV for 8 h, followed by immunoprecipitation with control rabbit-IgG or anti-MST4 antibodies. Lysates and immunoprecipitation extracts were probed with MST4 and MAVS antibodies. C Confocal microscopy of endogenous MST4 and MAVS in 293 T cells treated with SeV for 8 h. Scale bars, 10 µm. D Schematic representation of full-length MST4 and its truncated mutants. E Immunoblot analysis of lysates of 293 T cells transfected with Myc-MAVS and Flag-MST4 or its truncated mutants for 24 h, followed by immunoprecipitation with anti-Flag beads. F Schematic representation of full-length MAVS and its truncated mutants. G Immunoblot analysis of lysates of 293 T cells transfected with Flag-MST4 and HA-MAVS or its truncated mutants for 24 h, followed by immunoprecipitation with anti-Flag beads. H Luciferase activity in lysates of 293 T cells transfected with IFN-β-Luc and β-gal, along with Flag-MST4 or its truncated mutants, followed by the treatment with SeV for 8 h. Data information: The data are represented as mean ± SD (H: n = 3) and are representative of at least three independent experiments