Fig. 5

The LR-MSCs-myofibroblast transition was inhibited upon SENP1 downregulation in vitro and in vivo. A Representative images of dual staining in vitro for Collagen I (green) and α-SMA (red), SENP1 (green) and α-SMA (red), and dual staining for SENP1 (red) and Collagen I (green). Scale bar, 15 μm. B Collagen I, α-SMA, and SENP1 levels were assessed using western blotting analyses. Quantification is shown in the lower panel. C Representative images of dual staining in vivo for Sca-1 (green) and α-SMA (red), Sca-1 (green) and SENP1 (red). Scale bar, 50 μm. *P < 0.05, **P < 0.05, #P < 0.01, ##P < 0.01. *, control group vs. the TGFβ1 7d group. #, control group vs. the TGFβ1 14d group. **, the SENP1 knockdown group vs. the TGFβ1 7d group. ##, the SENP1 knockdown group vs. the TGFβ1 14d group. Each experiment was performed in triplicate