Fig. 4

The expression of SENP1 increases in LR-MSCs from mice. A LR-MSC morphology was observed using light microscopy after mouse LR-MSCs were treated with TGFβ1 (10 ng/mL) for 7 and 14 d. Black arrows indicate LR-MSCs. B Representative images of dual staining for Collagen I (green) and α-SMA (red), SENP1 (green) and α-SMA (red), and dual staining for SENP1 (red) and Collagen I (green). C Western blotting was applied to detect the levels of α-SMA, SENP1, Collagen I in LR-MSCs after the TGFβ1 (10 ng/mL) treatment for 14d. Scale bar, 20 μm. *P < 0.05, control group vs. the TGFβ1 7d group; **P < 0.01, control group vs. the TGFβ1 14d group. The results are shown as the means ± SD. Each experiment was performed in triplicate