Fig. 6

NHR-14 cooperates with CEP-1/p53 bind directly to the egl-1 promoter and regulate DNA damage-induced apoptosis. A Diagram of conserved NHR-14-binding DNA sequences. Most of the DNA fragment sequences bound to NHR-14 were analyzed by meme software. B Binding sites of NHR-14 and CEP-1 in the egl-1 promoter region. C Luciferase assay analysis of NHR-14 and CEP-1 directly bound to the egl-1 promoter region. D Quantitative analysis of germ cell apoptosis induced by gamma-irradiation at indicated time points post L4 in N2, nhr-14(tm1473), gld-1(op236) and gld-1(op236); nhr-14(tm1473). Up-regulated CEP-1/p53 via loss function of gld-1(op236) rescued DNA damage-induced programmed cell death in nhr-14(tm1473) animals. E The genetic pathway for nhr-14/HNF4 to regulate DNA damage-induced apoptosis. NHR-14/HNF4 functions as a cofactor of CEP-1/p53 to regulate DNA damage-induced apoptosis via controlling egl-1 and ced-13 expression