Fig. 4

nhr-14 is not a checkpoint gene and does not affect the cell cycle arrest after DNA damage. A Representative images of DAPI staining of the germline mitotic region in N2, nhr-14(tm1473) and hus-1(op244) worms. Young adult worms were treated with gamma-irradiation at 120 Gy. After 36 h of irradiation, germline was dissected and stained with DAPI. Bars, 5 μm. B Quantification of germline mitotic nuclear numbers after gamma irradiation in N2, nhr-14(tm1473), hus-1(op244), hus-1(op234); nhr-14(tm1473), clk-2(mn159), clk-2(mn159); nhr-14 (tm1473) worms. The gray and black bars represent nuclei numbers in the mitotic region in control and gamma irradiation-treated worm germline, respectively. *** indicate p < 0.001