Fig. 1

Inactivation of nhr-14/HNF4 inhibits DNA damage-induced programmed cell death in prmt-5(gk357). A Quantitative analysis of germ cell apoptosis in control RNAi- and nhr-14 RNAi-treated N2 and prmt-5(gk357) animals. N2 and prmt-5(gk357) were fed with control RNAi and nhr-14 RNAi and then (L4) was irradiated. After 36 h of gamma-irradiation, germ cell apoptosis from one gonad arm of each animal were scored from at least 20 animals. Error bars represent standard error of the mean (SEM). ** and *** indicate p < 0.01 and 0.001, respectively. B q-PCR analysis of the nhr-14 RNAi efficiency. C Quantitative analysis of germ cell apoptosis induced by gamma-irradiation in N2, nhr-14(tm1473), prmt-5(gk357) and prmt-5(gk357); nhr-14(tm1473). Germ cell apoptosis from one gonad arm of each animal were scored after 36 h of irradiation at indicated doses. At least 20 worms were scored at each radiation dose or time point. Error bars represent standard error of the mean (SEM). D Quantitative analysis germ cell apoptosis at indicated time points after irradiation (120 Gy) in N2, nhr-14(tm1473), prmt-5(gk357) and prmt-5(gk357); nhr-14(tm1473) animals. E. Quantitative analysis of germ cell apoptosis in ced-1(e1375); prmt-5(gk357) and ced-1(e1375); prmt-5(gk357); nhr-14(tm1473) animals with and without IR treatment. *** indicate p < 0.001. F. Time lapse analysis of germ cell corpse duration in N2 and nhr-14(tm1473) upon DNA damage