Fig. 6

Metabolic maturation of hiPSC-CMs. a and b RT-qPCR analysis of the glycolysis-associated genes (a) and fatty acid β-oxidation genes expression (b). c Traces and d quantification graph for OCR measured by Seahorse respirometry in hiPSC-CMs co-cultured with M0, M1, or M2 macrophages. The mRNA levels are shown relative to those of the M0 macrophage, which were set at 1. Data are presented as mean ± SEM, N = 3 individual preparations. Statistics were done by one-way ANOVA test with Bonferroni correction. *P < 0.05, **P < 0.01, ***P < 0.001. OCR, oxygen consumption rate