Fig. 3

Cardiac differentiation capacity of iPSCs co-cultured with M0, M1, and M2 macrophages. a Scheme showing the set-up of iPSCs cardiomyogenic differentiation in a monoculture or co-cultured with M0, M1, and M2 macrophages using a transwell system. Cardiac mesoderm precursors were first derived from iPSCs. Then the cardiac mesoderm precursors continued cardiomyogenic differentiation in a monoculture or co-cultured with M0, M1, and M2 macrophages using a transwell system. b Fluorescence image of iPSC-CM-Mono, iPSC-CM-M0, iPSC-CM-M1, and iPSC-CM-M2 stained for sarcomeric α-actinin (red). Cell nuclei were counterstained with Hoechst 33,342 (blue). c Quantification of cell nuclei in cultures with indicated conditions by ImageJ. iPSC-CM, iPSCs derived CM; iPSC-CM-Mono, iPSC derived CM in monoculture; iPSC-CM-M0, iPSCs derived CM in a co-culture with M0; iPSC-CM-M1, iPSCs derived CM in a co-culture with M1; iPSC-CM-M2, iPSCs derived CM in a co-culture with M2