Fig. 2

Cell proliferation of iPSCs co-cultured with M0, M1, or M2 macrophages. a Scheme showing the set-up of iPSCs proliferation in a monoculture or co-cultured with M0, M1, and M2 macrophages using a transwell system. b Fluorescence image of the cell nuclei of iPSCs monoculture or co-cultured for 3 days with M0, M1, and M2 macrophages stained with Hoechst 33,342 (blue). c Quantification of cell nuclei in iPSCs cultures with indicated conditions. d Cell viability analysis of iPSCs cultures with indicated conditions performed by CCK8. iPSC-Mono, iPSCs monoculture; iPSC-M0, iPSCs co-cultured with M0; iPSC-M1, iPSCs co-cultured with M1; iPSC-M2, iPSCs co-cultured with M2. Data are presented as mean ± SEM, N = 3 individual preparations. Statistics were done by one-way ANOVA test with Bonferroni correction. ***P < 0.001