Fig. 5

AKT is phosphorylated at CDRs in Hep3B cells. A and D Cytochalasin D (Cyto D) treatment completely blocked CDR formation in response to EGF (A) and insulin (D). **P < 0.01, ****P < 0.0001, by two-tailed Student’s t-test. B and E Cyto D attenuated AKT phosphorylation (pAKT) induced by EGF (B) or insulin (E). Cyto D did not affect ERK phosphorylation (pERK). C and F Quantification of pAKT/AKT ratios at the indicated times after EGF (C) and insulin (F) stimulations without (black) or with (red) Cyto D treatment from three independent experiments. Results are indicated as arbitrary units (AU). *P < 0.05, **P < 0.01, by one-tailed Student’s t-test. G and H Representative confocal images of actin-AKT (G) and AKT-pAKT after EGF or insulin treatments (3 min). A strong AKT signal (green) was observed at CDRs identified by actin staining (red) (G, enlarge images). Enlarged images of (H) show co-localization of AKT (red) and pAKT (green) at CDRs