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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Bone morphogenetic protein inhibitors and mitochondria targeting agents synergistically induce apoptosis-inducing factor (AIF) caspase-independent cell death in lung cancer cells

Fig. 4

Ym155 in combination with JL5 increases cytosolic AIF. A–D Western blot analysis of cytosol of A549 and H1299 cells treated with JL5 and Ym155 alone and in combination for 3 or 24 h. Arrowheads demonstrate the 67 kd and 57 kd fragments of AIF, which only occur in cells treated with JL5 and Ym155 in combination. E–F Western blot analysis of cells treated for 24 h demonstrating a decrease expression of anti-apoptotic proteins when cells are treated with JL5 and Ym155 in combination. G TUNEL assay of H1299 cells were treated with JL5 and Ym155 alone and in combination for 3 h. H TUNEL assay of H1299 and A549 cells treated with JL5 and Ym155 alone and in combination for 24 h. I TUNEL assay of H1299 cells treated with DMH2, SB-505124 (SB), alone or in combination with Ym155 for 24 h. G, H Data represents the mean of 4 independent experiments

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Cell Communication and Signaling

ISSN: 1478-811X