Fig. 5
β-catenin interacts with NICD and mediates TAK1 activation in macrophages. Bone marrow-derived macrophages (BMMs, 1 × 106) were cultured with or without LPS (100 ng/ml) for 6 h. A Immunofluorescence staining for macrophage β-catenin (green) and NICD (red) co-localization in LPS-stimulated macrophages. DAPI was used to visualize nuclei (blue). Scale bars, 30 μm. B Immunoprecipitation analysis of β-catenin and NICD in LPS-stimulated macrophages. C Western blot analysis of β-catenin, TRAF6, p-TAK1 and p-P65 in BMMs transfected with CRISPR/Cas9-mediated β-catenin activation and control. Representative of three experiments. D Western blot analysis of β-catenin, TRAF6, p-TAK1 and p-P65 in BMMs transfected with CRISPR/Cas9-mediated β-catenin KO and control. Representative of three experiments