Fig. 9
From: Inhibition of AKT induces p53/SIRT6/PARP1-dependent parthanatos to suppress tumor growth
The antitumor effect of AKT inhibition depends on p53 in vivo. Nude mice were injected s.c. with 1 × 106 HCT116 wt or p53–/–, and mice were treated with SC66 at 25 mg/kg by i.p. injection every 3 days for 15 days. a The body weight of mice in each group during treatment. b, c, d Representative tumors at the end of the experiment (b) Tumor weight (c) and tumor volume (d) were measured after treated with SC66. e, f Western blotting assay tested the levels of p53, p-AKT, ki67 (e), PAR polymer, cleaved-caspase3 and LC3 (f) in the harvested tumor. g Immunohistochemistry analysis the levels of PAR polymer and SIRT6 in the tumors. *p < 0.05