Fig. 4
From: Inhibition of AKT induces p53/SIRT6/PARP1-dependent parthanatos to suppress tumor growth
Blocking of PARP1 activity abrogated AKT related parthanatos. a Annexin V/PI staining and flow cytometry analysis of cell death under 10 μM SC66 treatment for 24 h with or without PARP1 inhibitor, 3AB (10 μM). b, c Cell viability of HCT116 was detected by CCK-8 assay after administrated 2 μM SC66 (b) and 10 μM TIC10 (c) for 48 h with or without 10 μM 3AB. d Immunofluorescent analysis of AIF (green) translocation under SC66 (10 μM) treatment with or without 3AB (10 μM). e, f The expressions of PAR polymer in HCT116 were tested after administrated SC66 with or without 3AB by western blotting assay (e) and immunofluorescent stain (f) of the PAR polymer (red). g Immunohistochemistry analysis shows PAR polymer expression in human CRC tissue and adjacent tissue. *p < 0.05