Fig. 3
From: Natural antisense RNA Foxk1-AS promotes myogenic differentiation by inhibiting Foxk1 activity
Foxk1-AS promoted myoblast differentiation by reducing the expression of Foxk1 in C2C12 cells. (A)(B) Overexpression of Foxk1-AS decreased the expression of the Foxk1 gene at the transcriptional level in C2C12 cells. (C) Overexpression of Foxk1-AS decreased the expression of the Foxk1 gene at the translational level in C2C12 cells. (D) Overexpression of Foxk1-AS increased the expression of the MyoG gene at the transcriptional level in C2C12 cells. (E) Overexpression of Foxk1-AS increased the expression of the MHC gene at the transcriptional level in C2C12 cells. (F) Overexpression of Foxk1-AS increased the expression of the MHC gene at the translational level in C2C12 cells. (G) Overexpression of Foxk1-AS increased MHC staining on Day 3 after skeletal muscle differentiation (left), and the fusion index was calculated (right). The fusion index was defined as the ratio of the number of nuclei in myotubes to the total number of nuclei