Fig. 6
From: Transforming growth factor-β1 negatively regulates SOCS7 via EGR1 during wound healing
EGR1 promotes re-epithelialization during wound healing through partial suppression of SOCS7 expression. A. Western blot analysis of TGF-β1, EGR1, and SOCS7 protein expression levels in C57BL/6 J mouse skin tissue at the wound edge at the indicated days after wounding. B. Representative immunohistochemical images of TGF-β1, EGR1, and SOCS7 staining in C57BL/6 J mouse skin tissue at the wound edge at days 0 and 7 post-wound-injury. C. Representative images showing wound healing over the course of 14 days post-wounding in mice treated with scrambled controls (siR-ctrl), EGR1 siRNA (siR-EGR1), or SOCS7 siRNA (siR-SOCS7) (left). Quantification of wound healing was carried out by calculating the wound area as a percentage of the initial wound area (right). D. Representative images of H&E-stained sections from C57BL/6 J mouse skin tissue on day 7 post-wound-injury showing the degree of re-epithelialization (left panel). The percentage of re-epithelialization (% wound closure) was determined using ImageJ software (right panel). Data are given as mean ± standard deviation (SD) from five mice. *P < 0.05; **P < 0.01; ***P < 0.001