Fig. 5

TGF-β1-induced keratinocyte motility is mediated through EGR1 and is partially dependent on the repression of SOCS7. A–D. HaCaT cells were co-transfected with EGR1 siRNA (siR-EGR1) or SOCS7 siRNA (siR-SOCS7) for 24 h, then treated with TGF-β1 (10 ng/ml) for 24 h. A. SOCS7 and EGR1 protein levels were analyzed by western blotting. B. Protein expression levels of phosphorylated ERK1/2 (p‑ERK1/2), total ERK, phosphorylated AKT (p‑AKT) and total AKT were analyzed by western blotting. C. Wound healing was assessed using the scratch‐wound assay. Representative images (left) and quantification of data showing the percentage of wound closure (right). D. Cell migration was assessed using the Transwell migration assay. Representative images showing crystal violet staining (left) and quantification of data showing the number of migrated cells/field (right). Data are given as mean ± standard deviation (SD) of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001