Fig. 4

Knockdown of EGR1 prevents TGF-β1-induced keratinocyte motility. A–B. EGR1 mRNA and protein levels in TGF-β1-treated HaCaT cells were analyzed by RT-PCR (A) and western blotting (B), respectively. C–F. HaCaT cells were transfected with scrambled siRNA (siR-ctrl) or EGR1 siRNA (siR-EGR1) for 24 h, then treated with TGF-β1 (10 ng/ml) for 24 h. EGR1 protein levels were analyzed by western blotting (C). SOCS7 protein levels were analyzed by western blotting (D). The scratch‐wound assay was used to examine the effect of EGR1 knockdown on TGF‐β1‐dependent wound healing. Representative images (left) and quantification of data showing the percentage of wound closure (right) (E). Cell migration was assessed using the Transwell migration assay. Representative images showing crystal violet staining (left) and quantification of data showing the number of migrated cells/field (right) (F). Data are given as mean ± standard deviation (SD) of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001