Fig. 1

SOCS7 is downregulated by TGF-β1 and mediates TGF-β1-induced keratinocyte migration and wound healing. A, B. SOCS7 mRNA and protein levels in TGF-β1-treated HaCaT cells were analyzed by RT-PCR (A) and western blotting (B), respectively. C–E. HaCaT cells were transfected with Vector or SOCS7 plasmids, and scrambled siRNA (siR-ctrl) or SOCS7 siRNA (siR-SOCS7) for 24 h, then treated with TGF-β1 (10 ng/ml) for 24 h. SOCS7 protein levels were analyzed by western blotting (C). The scratch‐wound assay was performed to analyze the effect of SOCS7 on TGF‐β1‐dependent cell migration. Representative images (left) and quantification of data showing the percentage of wound closure (right) (D). The Transwell migration assay was carried out to determine the effect of SOCS7 on TGF‐β1-dependent cell migration. Representative images showing crystal violet staining (left) and quantification of data showing the number of migrated cells/field (right) (E). Data are given as mean ± standard deviation (SD) of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001