Fig. 5

GW4064 inhibited the proliferation and apoptosis of CC cells via suppressing the Wnt-β-catenin signal pathway and FXR expression. a GW4064 inhibited the viability of the CC cells in a dose- and time-dependent manner. Living-cell image was used to detect the proliferative capability of CC cells. b, c Flow cytometry was performed to assess the apoptosis rate of different CC cells, and the apoptosis rate was higher in GW4064 than DMSO group. d qRT-PCR was used to detect the mRNA level of FXR, β-catenin, c-Myc, and cyclinD1 in different CC cells, and the expression level of FXR, β-catenin, c-Myc, and cyclinD1 were higher in DMSO than GW4064 groups. e Western blotting was used to detect the protein level of β-catenin, FXR, c-Myc, VGEF, cyclinD1, and CDK1 in different CC cells. f Western blotting was used to detect the protein level of cytosolic β-catenin in different CC cells. *P < 0:05, **P < 0.01, ***P < 0.001