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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Tumor-associated macrophages promote epithelial–mesenchymal transition and the cancer stem cell properties in triple-negative breast cancer through CCL2/AKT/β-catenin signaling

Fig. 4

TAMs promote β-catenin expression and activity in BT549 and HCC1937 cells. A Western blotting analysis for protein levels of total β-catenin and phosphorylated β-catenin at Ser675 and Ser552 in BT549 and HCC1937 cells cultured with M0-CM or M2-CM. B qRT-PCR analysis of β-catenin mRNA levels in BT549 and HCC1937 cells cultured with M0-CM or M2-CM. C Western blotting showing nuclear and cytoplasmic protein levels of β-catenin in BT549 and HCC1937 cells. D Immunofluorescence staining of β-catenin in BT549 and HCC1937 cells cultured with M0-CM or M2-CM. E The relative luciferase activity of TOPFlash and FOPFlash in BT549 and HCC1937 cells cultured with M0-CM or M2-CM. F–I BT549 and HCC1937 cells were transfected with β-catenin shRNA followed by cultured M2-CM or M0-CM. F The CD44+/CD24− cell populations were measured by flow cytometry. G The expression of β-catenin, SOX2, Nanog, and OCT4 were measured using western blotting. H Cell invasion were measured by transwell assay. I The expression of E-cadherin, N-cadherin, vimentin, and Snail was measured using western blotting. N = 3. *p < 0.05, **p < 0.01

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Cell Communication and Signaling

ISSN: 1478-811X