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Fig. 8 | Cell Communication and Signaling

Fig. 8

From: TGF-β1 regulates chondrocyte proliferation and extracellular matrix synthesis via circPhf21a-Vegfa axis in osteoarthritis

Fig. 8

Knockdown of Vegfa reversed overexpression of circPhf21a induced ECM degradation and proliferation inhibition in PMCs. A, B PMCs were transfected with negative control vector or oe-circPhf21a with or without si-Vegfa. After 48 h of transfection, the proliferation rate of PMCs was detected by EdU assay. n = 3, ***p < 0.001, ****p < 0.0001, scale bar = 100 μm. C PMCs were transfected with negative control vector or oe-circPhf21a with or without si-Vegfa. After 48 h of transfection, mRNA expression levels of Mmp13, Mmp3, Adamts4, Col2a1 and Aggrecan were detected by RT-qPCR analysis. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. D PMCs were transfected with negative control vector or oe-circPhf21a with or without si-Vegfa. After 48 h of transfection, the protein levels of Col2a1 and Mmp13 in PMCs were detected by western blotting. n = 3, ***p < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X