Table 1 Primers for real-time quantitative PCR
Gene | 5′–3′ | Amplicon size |
|---|---|---|
β-Actin | F: GTGGCCGAGGACTTTGATTG | 73 |
R: CCTGTAACAACGCATCTCATATT | ||
HK2 | F:CGACAGCATCATTGTTAAGGAG | 233 |
R: GCAGGAAAGACACATCACATTT | ||
PKM2 | F: ACTGGCATCATCTGTACCATTG | 84 |
R: AGCCACATTCATTCCAGACTTA | ||
LDHA | F: CCAATATGGCAACTCTAAAGGATC | 151 |
R: GCAAGTTCATCTGCCAAGTCCT | ||
Bcl2 | F: GACTTCGCCGAGATGTCCAG | 129 |
R: GAACTCAAAGAAGGCCACAATC | ||
Bax | F: CGAACTGGACAGTAACATGGAG | 157 |
R: CAGTTTGCTGGCAAAGTAGAAA | ||
hsa-miR-143-3p | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGAGCTA | 65 |
F: TGAGATGAAGCACTGTAGCTCGTC | ||
R: CAGTGCAGGGTCCGAGGTAT | ||
hsa-miR-155-5p | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACCCC | 65 |
F: GCGGCTCCTACATATTAGCATTAAC | ||
R: CAGTGCAGGGTCCGAGGTAT |