Fig. 3

Analysis of CAFs’ migratory and invasive abilities obtained in culture with melanoma-conditioned media (CM) or melanoma cells on Transwell inserts (INS). Control (CTRL) constitutes of fibroblasts cultured in FBM:DMEM (1:1 ratio) analogous to tested cells. To investigate migration and invasion A 2D and B 3D wound healing assays were performed. Relative wound density—determined using IncuCyte® Live-Cell Analysis System—is shown. Transwell invasion tests were also conducted (C). The number of control cells that invaded was set as 100%. The relative invasion factor was calculated. All experiments were conducted three times, each performed in triplicate and the mean ± SD is shown. Asterisks indicate statistically important differences between control cells and CAFs or between different types of CAFs. The significance level was set at p ≤ 0.05 (*), p ≤ 0.01 (**), p ≤ 0.001 (***), and p ≤ 0.0001 (****)