Fig. 2

Identification of CAFs. Western Blot analysis of protein level of FAP (A) in cell lysates and IL6 (B) in media collected from activated fibroblasts cultured with melanoma-conditioned media (CM) or with melanoma cells growing on Transwell inserts (INS). Signal was normalized to total protein content assessed by Ponceau S staining. Control (CTRL) constitutes fibroblasts cultured in FBM:DMEM (1:1 ratio) media analogous to tested cells. In the case of IL6, due to the lack of signal in CTRL, the fold is set vs signal from the last lane (Hs294T). The mean of at least three biological repetitions ± SD is shown. Asterisks indicate statistically important differences between control cells and CAFs or between different types of CAFs. The significance level was set at p ≤ 0.05 (*), p ≤ 0.01 (**), p ≤ 0.001 (***), and p ≤ 0.0001 (****)