Fig. 5

CCR1 upregulated in early OCPs in CRC microenvironment. A qRT-PCR analysis detected mRNA level of CCR1, CCR2 and CCR3 in early OCPs cultured in MC-38 CM. n = 3. B Western blotting analysis showed protein levels of CCR1 in early OCPs cultured in MC-38 CM or not (left) and the quantification of protein level normalized to β-actin (right). n = 3. C Transwell analysis showed migration of early OCPs transfected with CCR1 siRNA or scRNA in the presence of CCL7 (left) and quantification of migration number of early OCPs (right). n = 5. D Quantification of C using Absorbency analysis. n = 5. E qRT-PCR analysis detected mRNA level of CCR1 in early OCPs cultured in MC-38 CM with/without treatment of ERK antagonist or p38 MAPK antagonist. n = 3. F Western blotting analysis showed protein levels of CCR1 in early OCPs cultured in MC-38 with/without treatment of p38 MAPK antagonist or ERK antagonist (left) and the quantification of protein level normalized to β-actin (right). *p < 0.05, **p < 0.01, ***p < 0.001