Fig. 4

T cells from PKD3-deficient mice show a shift towards an effector/memory phenotype. T cell subpopulations from splenocytes of wild type and PKD3^−/− mice were analyzed by flow cytometry. a Frequencies of naïve and effector/memory T cells were distinguished by CD44 and CD62L staining. Representative FACS dot plots (gated for viable CD4⁺ and CD8⁺ lymphocytes) as well as summarizing graphs are shown for naïve (CD62L⁺CD44⁻) and effector/memory (CD62L⁻CD44⁺) CD4⁺ T cells as well as for naïve (CD62L⁺CD44⁻), central memory (CD62L⁺CD44⁺) and effector/memory (CD62L⁻CD44⁺) CD8⁺ T cells. b CD44⁺CD8⁺ T cells are further discriminated into memory precursor (KLRG1⁻CD127⁺) and short-lived effector (KLRG1⁺CD127⁻) cells. Data are represented as individual values plus mean from 4 independent experiments (n ≥ 9 mice; *p < 0.05, **p < 0.01, ***p < 0.001; two-tailed unpaired t-test)