Fig. 2
From: Addressing the role of PKD3 in the T cell compartment with knockout mice
In vivo response to immunization of PKD3-deficient mice. a An immunoblot showing that PKD3 protein is absent in PKD3−/− mice. b The pie charts illustrate the genotype distribution (wt; ± ; ko) of offspring from PKD3 heterozygous intercross (male in blue, and female in red colors). c–e The immune response of PKD3−/− and wild type mice was compared 7 days after intraperitoneal immunization with OVA/alum: c spleen weight; d frequency of splenic GC B cells and e Tfh cells, determined ex vivo by flow cytometric analysis. Data are represented as individual values plus mean from 4 independent experiments (n ≥ 11 mice; ns = not significant, **p < 0.01, ***p < 0.001; two-tailed unpaired t-test). Representative FACS dot plots of individual wt and PKD3−/− mice are shown