Fig. 1

PKD expression in CD4⁺ T lymphocytes. a Schematic depiction of the three PKD isoforms showing their conserved domains and phosphorylation sites. b Ex vivo MACS-sorted CD4⁺ T cells were stimulated for the depicted period of time with anti-CD3/CD28 antibodies, cross-linked by anti-hamster IgG, or P/I. Activating phosphorylation of PKDs’ serines in their activation loop was analyzed by SDS-PAGE and immunoblot. Actin was used as loading control. c PKD2 and PKD3 mRNA expression of CD4⁺ T cells isolated from wild type mice was analyzed after 2.5, 18 and 40 h of stimulation with platebound anti-CD3 and soluble anti-CD28 antibodies by qRT-PCR. The house keeping gene gapdh was used for normalization, and the results are depicted relative to unstimulated T cells ex vivo (set to one; highlighted by dashed line). d PKD2 and PKD3 protein expression of MACS-sorted wild type CD4⁺ T cells was analyzed upon in vitro stimulation for 18, 48 and 72 h by SDS-PAGE and immunoblot. Actin was used as loading control