Fig. 7

ACT001 promoted angiogenesis of bEnd.3 in cell interaction models. a Schematic diagram of BV2-bEnd.3 cell interaction model structure. bEnd.3 cells were seeded in each Matrigel-coated well and co-cultured with pre-stimulated BV2 cells for 24 h. b The tube formation of bEnd.3 cells after co-culturing with BV2 cells for 24 h without ACT001 and LPS treatment were shown as Control. Scale bar = 150 μm. b (cont) After co-culturing with 100 ng/ml LPS (B, up panel) or 500 ng/ml LPS (b, down panel) pre-stimulated BV2 cells for 24 h, the tube formation of bEnd.3 cells decreased markedly. Conversely, ACT001 treatment could reverse this effect and promote the angiogenesis in a dose-dependent manner. Scale bar = 150 μm. c The VEGF expression in bEnd.3 and  LPS-stimulated BV2 cells co-cultured medium was analyzed by ELISA assays. d Representative western blot results of ZO-1 and Occludin in bEnd.3 cells after co-culturing for 24 h. Actin was used as control for protein loading. * P < 0.05, **P < 0.01, ***P < 0.001 versus Con trol group; # P < 0.05, ## P < 0.01, ### P < 0.00 1 versus LPS group