Fig. 6

ACT001 reversed HT22 cells apoptosis induced by activated BV2 cells. a Schematic diagram of BV2-HT22 cell interaction model structure. BV2 cells were pre-treated with LPS for 24 h, then switched into models and co-cultured with HT22 cells in ACT001-added complete DMEM for 24 and 48 h. b After co-culturing for 24 and 48 h, the NO production levels in supernatants of cell interaction models were determined by using Griess reagent. c Representative fluorescence images of NeuN staining with TUNEL labelling in HT22 cells after co-culturing in models for 24 h. Cell nuclei were shown in blue (DAPI). Scale bar = 100 μm. d After co-culturing for 24 h, HT22 cells apoptosis were analyzed through flow cytometry assay by Annexin V-FITC/PI dual staining. Cell interaction models without ACT001 and LPS treatment were shown as Control. Data were presented as means ± SEMs of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 versus Control group; # P < 0.05, ## P < 0.01, ### P < 0.001 versus ACT001 0 μM + LPS group