Fig. 1

ACT001 attenuated neurological impairment and promoted neurobehavioral recovery in mice CCI models. a Overview of experimental timeline for in vivo studies and neurobehavioral tests. b Surgical process illustration of CCI models. After contusion injury (left), the bone flap was sealed with sterile bone wax (middle), indicated by orange arrow. The peri-contusional tissue region at 14 days post-insult was shown on the right panel. c Representative photographs of whole brains in Sham, TBI and TBI + ACT001 groups at 3 and 7 days post-insult. The circles represented the approximate area of lesion cortex. d Representative cresyl violet-stained brain sections at indicated time points post-insult. n = 6/group. e Representative images of EB extravasation in three groups at indicated time points post-insult (blue areas indicated the dye extravasation). n = 6/group. f-i Line graphs showed four neurobehavioral function assessments at indicated time points including mNNS scores (f), Grid-Walking test (g), Rotarod test (h) and Hanging Wire test (i). n = 8/group. Data were presented as mean ± SEMs, *P < 0.05, **P < 0.01, versus TBI group