Fig. 3

The neuroprotective role of CGA in hypoxic-sschemic brain injury through activation of Sirt1 to regulate the Nrf2-NF-κB signaling pathway. a Representative results from Western blot showing the protein levels of Sirt1, IκB, and HO-1 in cytoplasm, Nrf2 and NF-κB in nucleus 24h after HI injury. b–f Quantification of western blot data of Sirt1, IκB, HO-1, Nrf2, NF-κB. ∗P < 0.05, ∗∗P < 0.01 and ∗∗∗P < 0.001 vs. the sham group. #P < 0.05, ##P < 0.01 and ###P < 0.001 vs. the HI group. n = 3. g Representative results from Western blot showing the protein levels of NF-κB and p-NF-κB in the cytoplasm 24h after HI injury. h Representative results from Western blot showing the protein levels of NF-κB, p-NF-κB and Nrf2 in the nuclear 24h after HI injury. i Representative results from Western blot showing the protein levels of NF-κB and p-NF-κB in the whole protein 24h after HI injury. j–k Quantification of western blot data of Nrf2, NF-κB in the nuclear. ∗P < 0.05, ∗∗P < 0.01 vs. the sham group. #P < 0.05, ##P < 0.01 vs. the HI group. &P < 0.05, vs. the CGA pretreatment group. n = 3