Fig. 1

Bone marrow-specific deficiency of AIBP aggravates atherosclerosis in LDLR^−/− mice. A Representative images showing Mac3 and AIBP staining in human samples obtained from coronary artery plaques (n = 3). Scale bar = 1000 μm. B Western blot analysis of the AIBP protein level in mouse atherosclerotic plaques. β-Actin bands indicate the loading control (n = 5). C Western blot analysis of AIBP levels in BMDMs from AIBP^WT/LDLR^−/− and AIBP^∆BMK/LDLR^−/− mice. β-Actin bands indicate the loading control (n = 5). D qPCR analysis of the AIBP mRNA levels in BMDMs from AIBP^WT/LDLR^−/− and AIBP^∆BMK/LDLR^−/− mice. E Body weights of AIBP^WT/LDLR^−/− and AIBP^∆BMK/LDLR^−/− mice fed a HFD for 8 weeks (n = 5). F Comparison of the composition of serum lipids between AIBP^WT/LDLR^−/− and AIBP^∆BMK/LDLR^−/− mice fed a HFD for 8 weeks. TC (total serum cholesterol), TG (triglyceride), LDL (low-density lipoprotein) and HDL (high-density lipoprotein) contents were assayed (n = 5). G Oil Red O staining of aortic trees from AIBP^WT/LDLR^−/− and AIBP^∆BMK/LDLR^−/− mice. H Cross-sections of aortic roots from mice stained with hematoxylin and eosin (H&E) and Oil Red O (n = 10). Scale bar = 100 μm. Data are presented as the means ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001. Unpaired two-tailed Student’s t test