Fig. 6

tRF-Val-CAC-016 was immunoprecipitated by Argonaute-2 and could modulate the proliferation of GC by targeting CACNA1d. a Binding relation between tRF-Val-CAC-016 and CACNA1d mRNA. b–c tRF-Val-CAC-016 mimics could significantly reduce the expression of CACNA1d using RT-PCR and immunoblotting. d Dual-luciferase reporter assay indicated that WT-CACNA1d-3′UTR plus tRF-Val-CAC-016 mimics group could significantly reduce the luciferase ratio compared with other groups in NCI-N87 and HGC-27. e In RIP assay, tRF-Val-CAC-016 was significantly immunoprecipitated by Argonaute-2 compared with the IgG group. f–g Gel-electrophoresis further verified the PCR product of RIP assays, and the immunoblotting confirmed the integrity of the process to wash the magnetic beads. *P < 0.05, **P < 0.01, statistically significant